王存等《New Phytologsit》2024年

论文题目：CPK10 protein kinase regulates Arabidopsis tolerance to boron deficiency through phosphorylation and activation of BOR1 transporter

论文作者：Zhangqing Wang^#, Yanting Zhang^#, Yaru Wu, Duoduo Lai, Yuan Deng, Chuanfeng Ju, Lv Sun, Panpan Huang* and Cun Wang*

论文摘要： Boron (B) is crucial for plant growth and development. B deficiency can impair numerous physiological and metabolic processes, particularly in root development and pollen germination, seriously impeding crop growth and yield. However, the molecular mechanism underlying boron signal perception and signal transduction is rather limited. In this study, we discovered that CPK10, a calcium-dependent protein kinase in the CPK family, has the strongest interaction with the boron transporter BOR1. Mutations in CPK10 led to growth and root development defects under B-deficiency conditions, while constitutively active CPK10 enhanced plant tolerance to B deficiency. Furthermore, we found that CPK10 interacted with and phosphorylated BOR1 at the Ser689 residue. Through various biochemical analyses and complementation of B transport in yeast and plants, we revealed that Ser689 of BOR1 is important for its transport activity. In summary, these findings highlight the significance of the CPK10-BOR1 signaling pathway in maintaining B homeostasis in plants and provide targets for the genetic improvement of crop tolerance to B-deficiency stress.

硼对植物生长发育至关重要，缺硼会影响植物的多种生理和代谢过程，特别是根系发育和花粉萌发，严重阻碍作物的生长和产量。然而，植物对于硼信号感知和信号转导的分子机制尚不清楚。本研究发现CPKs家族中的钙依赖性蛋白激酶CPK10与硼转运体BOR1的相互作用最强，并且在缺硼条件下，CPK10的突变导致生长和根系发育缺陷，而组成型活性CPK10增强了植物对缺硼的耐受性。此外，CPK10与BOR1的Ser689残基相互作用并磷酸化。通过对酵母和植物中硼转运的各种生化分发现，BOR1的Ser689对其转运活性至关重要。综上所述，该研究揭示了CPK10-BOR1信号通路在维持植物硼稳态中的重要意义，并为作物耐缺硼胁迫的遗传改良提供了靶点。

论文链接：http://doi.org/10.1111/nph.19712