张斌、麻鹏达、董娟娥等《Journal of Experimental Botany》2023年
论文题目：Salicylic acid regulates phenolic acid biosynthesis via SmNPR1-SmTGA2/SmNPR4 modules in Salvia miltiorrhiza
论文作者：Meiling Ding, Yongfeng Xie, Yuhang Zhang, Xiaona Cai, Bin Zhang, Pengda Ma, Juane Dong
论文摘要：Phenolic acids are the main active ingredients in Salvia miltiorrhiza, which can be used for the treatment of many diseases, particularly cardiovascular diseases. It is known that salicylic acid (SA) can enhance phenolic acid content, but the molecular mechanism of its regulation is still unclear. Nonexpresser of PR genes 1 (NPR1) plays a positive role in the SA signaling pathway. In this study, we identified a SmNPR1 gene that responds to SA induction and systematically investigated its function. We found that SmNPR1 positively affected phenolic acid biosynthesis. Then, we identified a novel TGA transcription factor, SmTGA2, which interacts with SmNPR1. SmTGA2 positively regulates phenolic acid biosynthesis by directly up-regulating SmCYP98A14 expression. After double-gene transgenic analysis and other biochemical assays, it was found that SmNPR1 and SmTGA2 work synergistically to regulate phenolic acid biosynthesis. In addition, SmNPR4 forms a heterodimer with SmNPR1 to inhibit the function of SmNPR1, and SA can alleviate this effect. Collectively, these findings elucidate the molecular mechanism underlying the regulation of phenolic acid biosynthesis by SmNPR1-SmTGA2/SmNPR4 modules and provide novel insights into the SA signaling pathway regulating plant secondary metabolism.
酚酸是丹参的主要活性成分，可用于治疗多种疾病，特别是心血管疾病。已知水杨酸(SA)可提高酚酸含量，但其调控的分子机制尚不清楚。非表达者PR基因1 (NPR1)在SA信号通路中发挥积极作用。在这项研究中，我们鉴定了一个响应SA诱导的SmNPR1基因，并系统地研究了它的功能。我们发现SmNPR1正影响酚酸的生物合成。然后，我们确定了一种新的TGA转录因子SmTGA2，并发现它与SmNPR1相互作用。SmTGA2通过直接上调SmCYP98A14的表达，正向调节酚酸生物合成。通过双基因转基因分析和其他生化实验，发现SmNPR1和SmTGA2协同调节酚酸生物合成。此外，SmNPR4与SmNPR1形成异源二聚体，抑制SmNPR1的功能，SA可以缓解这种作用。总的来说，这些发现阐明了SmNPR1-SmTGA2/SmNPR4模块调控酚酸生物合成的分子机制，并为SA信号通路调控植物次生代谢提供了新的见解。