赵天永《The Plant Journal》2019

　　论文题目：Maize HSFA2 and HSBP2 antagonistically modulate raffinose biosynthesis and heat tolerance in Arabidopsis

　　论文作者：Lei Gu ，Tao Jiang ，Chunxia Zhang ，Xudong Li ，Chunmei Wang ，Yumin Zhang ，Tao Li ，Lynnette M.A. Dirk ，A. Bruce Downie ，Tianyong Zhao　　

　　论文摘要：Raffinose is thought to play an important role in plant abiotic stress tolerance. We report here that maize HEAT SHOCK FACTOR A2 (ZmHSFA2) and HEAT SHOCK BINDING PROTEIN 2 (ZmHSBP2) physically interact with each other and antagonistically modulate GALACTINOL SYNTHASE2 (ZmGOLS2) expression and raffinose biosynthesis in transformed maize protoplasts and Arabidopsis plants. Overexpression of ZmHSFA2 in Arabidopsis increased the expression of Arabidopsis AtGOLS1, AtGOLS2 and AtRS5 (RAFFINOSE SYNTHASE), increased the raffinose content in leaves, and enhanced the plant heat stress tolerance. Contrary to ZmHSFA2, overexpression of ZmHSBP2 in Arabidopsis decreased AtGOLS1, AtGOLS2 and AtRS5 expression, decreased raffinose contents in leaves, and reduced the plant heat stress tolerance. ZmHSFA2 and ZmHSBP2 also interact with their Arabidopsis counterparts AtHSBP and AtHSFA2 as determined using BiFC assays. Furthermore, endogenous ZmHSBP2 and Rluc, controlled by the ZmHSBP2 promoter, are transcriptionally activated by ZmHSFA2 and inhibited by ZmHSBP2 in maize protoplasts. These findings provide insights into the transcriptional regulation of raffinose biosynthetic genes, and the tolerance their product confers, to plant heat stress.

　　论文链接：https://onlinelibrary.wiley.com/doi/10.1111/tpj.14434