郁飞等《The EMBO Journal》2022年

论文题目：ARF2-PIF5 interaction controls transcriptional reprogramming in the ABS3-mediated plant senescence pathway

论文作者：Hui Xue, Jingjing Meng, Pei Lei, Yongxin Cao, Xue An, Min Jia, Yan Li, Haofeng Liu, Jen Sheen, Xiayan Liu, Fei Yu

论文摘要：One of the hallmarks of plant senescence is the global transcriptional reprogramming coordinated by a plethora of transcription factors (TFs). However, mechanisms underlying the interactions between different TFs in modulating senescence remain obscure. Previously, we discovered that plantABS3subfamily MATE transporter genes regulate senescence and senescence-associated transcriptional changes. In a genetic screen for mutants suppressing the accelerated senescence phenotype of the gain-of-function mutantabs3-1D, AUXIN RESPONSE FACTOR 2 (ARF2) and PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) were identified as key TFs responsible for transcriptional regulation in the ABS3-mediated senescence pathway. ARF2 and PIF5 (as well as PIF4) interact directly and function interdependently to promote senescence, and they share common target genes such as key senescence promoting genesORESARA 1(ORE1) andSTAY-GREEN 1(SGR1) in the ABS3-mediated senescence pathway. In addition, we discovered reciprocal regulation between ABS3-subfamily MATEs and the ARF2 and PIF5/4 TFs. Taken together, our findings reveal a regulatory paradigm in which the ARF2-PIF5/4 functional module facilitates the transcriptional reprogramming in the ABS3-mediated senescence pathway.

植物衰老的标志之一是由大量转录因子协调的全局转录重编程。然而，不同转录因子之间在调节衰老方面相互作用的机制仍然不清楚。此前，我们发现植物ABS3亚家族MATE转运蛋白基因调控衰老和衰老相关的转录变化。通过大规模遗传逆转突变筛选，发现两个不同家族的转录因子基因ARF2和PIF5突变可以有效逆转ABS3的功能获得型突变体abs3-1D的早衰和衰老相关基因异常激活表达的表型。ARF2和PIF5/4同时以相互依赖和各自独立的方式，共同控制ABS3下游衰老过程的转录调控。ARF2和PIF5/4直接互作，并且共同调控衰老途径中的靶基因，如关键衰老促进因子ORESARA 1（ORE1）和STAY-GREEN 1（SGR1）是二者共同作用的靶基因。不仅如此，我们还发现了ABS3亚家族MATE与ARF2和PIF5/4之间的相互调节关系。这些工作揭示了ARF2-PIF5/4功能模块调控植物ABS3介导衰老途径的分子范式。

论文链接：https://www.embopress.org/doi/full/10.15252/embj.2022110988