许晓东
许晓东
一、个人简介
许晓东,男,博士,教授,博士生导师,1967年10月出生,黑龙江省甘南县人。1989年在北京农业大学农业微生物专业获学士学位,1995年在北京农业大学微生物学专业获硕士学位,2009年在英国雷丁大学(University of Reading)病毒学专业获博士学位。2009年11月到西北农林科技大学英国正版365官方网站工作。主讲本科生“分子生物学”等课程。
二、研究方向
1. 杆状病毒的分子生物学。包括与病毒晚期基因表达相关的调控因子、病毒与宿主细胞的互作等领域的研究。
2. 朊病毒。近期我们在杆状病毒中发现了病毒中的第一例朊病毒——LEF10。我们推测其他种类的病毒中也存在朊病毒,因此我们拟用实验室已经建立的方法广泛筛选朊病毒。
实验室条件:能完整地实现PCR获得目的基因、克隆到相应的表达载体、原核或真核表达目标蛋白、蛋白质检测、蛋白质间相互作用、重组杆状病毒构建以及通过流式细胞术对细胞进行统计学分析。目前除了一个通用实验室之外,我们还建有一个具备二氧化碳培养箱、倒置显微镜等设备的动物细胞培养室,用于昆虫细胞和哺乳动物细胞培养室。
研究生要求:由于实验室的研究方向涉及广泛的分子生物学知识,因而需要具有坚实的分子生物学理论基础和动手能力、热爱科学研究并具有创新热情的同学加盟。
三、工作简历
2001.11-2009.10 英国雷丁大学(University of Reading),Technician, Research Assistant。 从事杆状病毒表达系统、单链抗体(scFv)、HIV和口蹄疫基因工程疫苗的研究。
1995.07-2000.07 中国科学院微生物研究所,科技处职员、副处长,综合办公室主任。
1989.07-1992.08 黑龙江省牡丹江制药厂,助理工程师。
四、科研成果
1. Nan H, Chen H, Tuite MF, Xu X. 2019 A viral expression factor behaves as a prion. Nat Commun. 2019 Jan 21;10(1):359. doi: 10.1038/s41467-018-08180-z.
2. Nan H, Lan J, Tian M, Dong S, Tian J, Liu L, Xu X, Chen H. 2018 The Network of Interactions Among Porcine Reproductive and Respiratory Syndrome Virus Non-structural Proteins. Front Microbiol. 2018 May 14;9:970. doi: 10.3389/fmicb.2018.00970.
3. Zhang X, Xu K, Ou Y, Xu X, Chen H. 2018 Development of a baculovirus vector carrying a small hairpin RNA for suppression of sf-caspase-1 expression and improvement of recombinant protein production. BMC Biotechnol. 18(1):24. doi: 10.1186/s12896-018-0434-1.
4. Liu H, Rahman SU, Mao Y, Xu X, Tao S. 2017 Codon usage bias in 5' terminal coding sequences reveals distinct enrichment of gene functions. Genomics. 109(5-6):506-513. doi: 10.1016/j.ygeno.2017.07.008.
5. Chen J, Xu X, Tao H, Wang Y, Chen H. 2016 Chemical shift assignments of nsp7α from porcine reproductive and respiratory syndrome virus. Biomol NMR Assign. 2016 Oct;10(2):391-4. doi: 10.1007/s12104-016-9706-7.
6. Xu X, Zhou X, Nan H, Zhao Y, Bai Y, Ou Y, Chen H. 2016 Aggregation of AcMNPV LEF-10 and Its Impact on Viral Late Gene Expression. PLoS One. 11(5):e0154835. doi: 10.1371/journal.pone.0154835.
7. Liu L, Tian J, Nan H, Tian M, Li Y, Xu X, Huang B, Zhou E, Hiscox JA, Chen H. 2016 Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Interacts with Nsp9 and Cellular DHX9 To Regulate Viral RNA Synthesis. J Virol. 90(11):5384-5398. doi: 10.1128/JVI.03216-15.
8. Wang Y, Mao Y, Xu X, Tao S, Chen H. 2015 Codon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System. PLoS One. 10(12):e0145887. doi:10.1371/journal.pone.0145887.
9. Wu F, Peng K, Tian J, Xu X, Zhou E, Chen H. 2014 Immune response to Fc tagged GP5 glycoproteins of porcine reproductive and respiratory syndrome virus. Viral Immunol. 27(7):343-9. doi: 10.1089/vim.2014.0041.
10. Liu B, Wang R, Wu F, Xu X, Chen H. 2014 Rapid production of HIV-1 neutralizing antibodies in baculovirus infected insect cells. Protein Expr Purif. 99:87-93. doi: 10.1016/j.pep.2014.04.004.
11. Xu X, Chen Y, Zhao Y, Liu X, Dong B, Jones IM, Chen H. 2013 Baculovirus superinfection: a probable restriction factor on the surface display of proteins for library screening. PLoS One. 8(1):e54631. doi: 10.1371/journal.pone.0054631
12. Porta C, Xu X, Loureiro S, Paramasivam S, Ren J, Al-Khalil T, Burman A, Jackson T, Belsham GJ, Curry S, Lomonossoff GP, Parida S, Paton D, Li Y, Wilsden G, Ferris N, Owens R, Kotecha A, Fry E, Stuart DI, Charleston B, Jones IM. 2013 Efficient production of foot-and-mouth disease virus empty capsids in insect cells following down regulation of 3C protease activity. J Virol Methods. 187:406-12
13. Kong L, Sheppard NC, Stewart-Jones GB, Robson CL, Chen H, Xu X, Krashias G, Bonomelli C, Scanlan CN, Kwong PD, Jeffs SA, Jones IM, Sattentau QJ. 2010 Expression-system-dependent modulation of HIV-1 envelope glycoprotein antigenicity and immunogenicity. J Mol Biol. 15:131-47
14. Chen H, Xu X, Lin HH, Chen SH, Forsman A, Chapman MA, Jones IM. 2008 The immune response to the outer domain of a HIV-1 clade C gp120 is predominantly V3 specific and includes neutralising antibodies. J. Gen. Virol. 89:2597-2604
15. Mathewson AC, Bishop A, Yao Y, Kemp F, Ren J, Chen H, Xu X, Berkhout B, van der Hoek L, Jones IM. 2008 Interaction of severe acute respiratory syndrome-coronavirus and NL63 coronavirus spike proteins with angiotensin converting enzyme-2. J. Gen. Virol. 89:2741-2745
16. Chen H, Xu X, Jones IM. 2007 Immunogenicity of the outer domain of a HIV-1 clade C gp120. Retrovirology 4:33
17. Chen H, Xu X, Bishop A, Jones IM. 2005 Reintroduction of the 2G12 epitope in an HIV-1 clade C gp120. AIDS 19(8):833-835
18. Xu X, Jones IM. 2004. Rapid parallel expression in E. Coli and insect cells: analysis of five lef gene products of the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). Virus Genes. 29(2):191-197
19. Tan Z, Xu X, and Chen W. 1997 Phylogenetic and Genetic Relationships of Mesorhizobium tianshanense and Related Rhizobia. Int. J. Syst. Bacteriol. 47(3):874-879
20. 许晓东, 陈文新. 1996 天山根瘤菌(Rhizobium tianshanense)全细胞蛋白电泳和多位点酶电泳分析. 微生物学通报. 23(3):131-134
五、联系方式
联系地址:712100 陕西杨凌西农路22号 西北农林科技大学英国正版365官方网站(理科楼D212)
电子邮件:xuxd@nwsuaf.edu.cn